Penyelidikan yang dilaporkan oleh penyelidik di Universiti Fudan menunjukkan bahawa gabungan baru empat biomarker protein boleh mendiagnosis dengan tepat barah pankreas. (Br J Cancer. Versi dalam talian 9 November 2017).
The researchers said that we believe that the development of a fast and stable method for screening target proteins in large samples may accelerate the pace of finding new protein biomarkers and drug targets, and may even be individualized in the future. Of proteomes are used in precision medicine.
jambatan kanser pankreas berada pada peringkat lanjut penyakit pada masa diagnosis, dan kadar kemandirian median selepas diagnosis adalah kurang daripada 6%. Diagnosis dan rawatan awal boleh meningkatkan prognosis.
The researchers used a variety of mass spectrometry techniques to analyze 150 serum samples from healthy controls, patients with benign pancreatic diseases, and patients with pancreatic cancer, and identified 142 differentially expressed proteins. Finally, the four proteins were included in their biomarker expression profiles: APOE , ITIH3, APOA1 and APOL1.
Through the analysis of the area under the curve (AUC) method, the accuracy of a single protein marker used to distinguish pancreatic cancer patients from healthy controls was between 66.9% and 89.6%. The combination of the four markers can increase the accuracy to 93.7%. The sensitivity of the four-protein biomarker combination in the diagnosis of pancreatic cancer was 85%, and the specificity was 94.1%. If CA19-9 is included in this detection method, the AUC will be increased to 0.99, at which time the sensitivity is 95% and the specificity is 94.1%.
Para penyelidik juga menggunakan imunohistokimia untuk mengesahkan ungkapan penanda protein di atas dalam sampel tumor, seterusnya mengesahkan kebolehpercayaan gabungan biomarker baharu ini.
Para penyelidik berkata bahawa aplikasi klinikal biomarker ini masih mempunyai perjalanan yang panjang. FDA AS baru sahaja meluluskan gabungan penanda ujian analisis tumor dari Pusat Kanser Memorial Sloan Kettering yang dipanggil IMPACT. IMPACT boleh mengesan mutasi dengan cepat dalam 468 gen dan perubahan molekul lain dalam komposisi genom tumor manusia.
