በፉዳን ዩኒቨርሲቲ ተመራማሪዎች የተዘገበው ጥናት እንደሚያሳየው አዲስ የአራት ፕሮቲን ባዮማርከርስ ጥምረት በትክክል መመርመር ይችላል። የጣፊያ ካንሰር. (Br J Cancer. የመስመር ላይ እትም ኖቬምበር 9, 2017)።
The researchers said that we believe that the development of a fast and stable method for screening target proteins in large samples may accelerate the pace of finding new protein biomarkers and drug targets, and may even be individualized in the future. Of proteomes are used in precision medicine.
አብዛኞቹ የጣፊያ ነቀርሳዎች በምርመራው ወቅት የበሽታው ከፍተኛ ደረጃ ላይ ናቸው, እና ከምርመራው በኋላ ያለው መካከለኛ የመዳን መጠን ከ 6% ያነሰ ነው. ቅድመ ምርመራ እና ህክምና ትንበያዎችን ሊያሻሽል ይችላል.
The researchers used a variety of mass spectrometry techniques to analyze 150 serum samples from healthy controls, patients with benign pancreatic diseases, and patients with pancreatic cancer, and identified 142 differentially expressed proteins. Finally, the four proteins were included in their biomarker expression profiles: APOE , ITIH3, APOA1 and APOL1.
Through the analysis of the area under the curve (AUC) method, the accuracy of a single protein marker used to distinguish pancreatic cancer patients from healthy controls was between 66.9% and 89.6%. The combination of the four markers can increase the accuracy to 93.7%. The sensitivity of the four-protein biomarker combination in the diagnosis of pancreatic cancer was 85%, and the specificity was 94.1%. If CA19-9 is included in this detection method, the AUC will be increased to 0.99, at which time the sensitivity is 95% and the specificity is 94.1%.
ተመራማሪዎቹ ከዚህ በላይ የተጠቀሱትን የፕሮቲን ምልክቶች በእብጠት ናሙናዎች ውስጥ መግለጻቸውን ለማረጋገጥ ኢሚውኖሂስቶኬሚስትሪን ተጠቅመዋል፣ ይህም አዲሱ የባዮማርከር ጥምረት አስተማማኝነት የበለጠ መሆኑን ያረጋግጣል።
ተመራማሪዎቹ የእነዚህ ባዮማርከርስ ክሊኒካዊ አተገባበር አሁንም ረጅም መንገድ እንደሚቀረው ተናግረዋል. የዩኤስ ኤፍዲኤ (ኤፍዲኤ) ከመታሰቢያ ስሎአን ኬተርንግ ካንሰር ማእከል ኢምፓክት (IMPACT) የተባለውን የዕጢ ትንተና መመርመሪያ ማርከርን አሁን አጽድቋል። IMPACT በ 468 ጂኖች ውስጥ ሚውቴሽን እና ሌሎች በሰው እጢ ጂኖም ስብጥር ላይ ያሉ ሞለኪውላዊ ለውጦችን በፍጥነት ማወቅ ይችላል።
