Ngenxa yokuthi umdlavuza wesibindi unezinhlobo eziningi, ifa eliqinile, nokuphindaphinda kalula, ukukhomba ama-biomarker angabikezela ukuqhubeka kwesifo kuwumgomo oyinhloko ekulweni nomdlavuza wesibindi.
Muva nje, abacwaningi sebesungule indlela yokuhlonza uhlobo oluvame kakhulu lomdlavuza wesibindi-hepatocellular carcinoma (HCC) ngokususelwa kuma-biomarker okusongela. Bakholelwa ukuthi le ndlela ingasetshenziselwa nezinye izinhlobo zomdlavuza. Lolu cwaningo luqhakambisa ukuthi ukwahlukahluka kwe-RNA splicing variants kunomthelela kanjani kumdlavuza, futhi kukhomba ukuthi lokhu okwehlukile kungaba yizimpawu zokuqhamuka komdlavuza.
Ukusika kusho inqubo lapho imininingwane ye-RNA ekopishwe kusuka kulwazi olufakwe kufuzo ihlelwa ngaphambi kokuthi isetshenziselwe ukwenza ibalazwe elithile lamaprotheni. Isakhi sofuzo singakhipha imiyalezo eminingi ye-RNA, futhi umyalezo ngamunye ukhiqiza okuhlukile kwamaprotheni noma “isomer.” Izifo eziningi zihlobene namaphutha noma ukuhluka ezindleleni zokusika ze-RNA. Amaphutha noma ushintsho ekusikeni kungadala amaprotheni anemisebenzi ehlukile noma engajwayelekile.
Recent research has identified splicing irregularities in umdlavuza wesibindi cells. Krainer’s team has developed a method that can comprehensively analyze all RNA information produced by a given gene. The team tested their methods of detecting splice variants in HCC by analyzing RNA information from HCC cells collected from hundreds of patients.
They found that the specific splicing isoform of the AFMID gene is associated with the patient’s low survival. These variants result in cells making truncated versions of the AFMID protein. These unusual proteins are associated with mutations in TP53 and ARID1A isisu suppressor genes in adult liver cancer cells.
Abacwaningi bacabange ukuthi lezi zinguquko zihlobene nezinga eliphansi le-molecule ebizwa nge-NAD +, ebandakanyeka ekulungiseni i-DNA eyonakele. Ukulungisa ukuxhumanisa kwe-AFMID kungaholela ekwandeni kokukhiqizwa kwe-NAD + kanye nokwanda kokulungisa i-DNA. Uma singakwenza lokhu, ukuthunga kwe-AFMID kungaba inhloso yokwelapha kanye nomthombo wemithi emisha yomdlavuza wesibindi. Ukuhlola kokuqala kubonisa ukuthi ucwaningo lwethimba lukumzila olungile, futhi silindele imiphumela engcono yedatha ukuze kuzuze iziguli ezinomdlavuza wesibindi.
