April 2022: Kazhinji, nzira yekugadzira sero yeCAR T-cell therapy inotora mazuva mapfumbamwe kusvika gumi nemana; zvisinei, vatsvakurudzi vepaYunivhesiti yePennsylvania vakakwanisa kugadzira masero eCAR T anoshanda pamwe nekuwedzera anti-bundu kushanda mumaawa 24 chete vachishandisa nzira itsva.
Rudzi rutsva rwe immunotherapy runodaidzwa kuti autologous CAR T-cell therapies inoshandisa murwere ega T masero, anoachinja kunze kwemuviri nekuwedzera CAR gene inoita kuti vataure mareceptors anonangisa zvirinani maseru egomarara, obva aadzosera mumurwere. . Mishonga iyi, kune rumwe rutivi, ine mukurumbira wakashata kwenguva refu yekugadzira, iyo inogona kukanganisa kukwanisa kwesero kuti idzokorore uye nokudaro kuderedza kurapa potency, tisingarevi kunokonzera varwere vanorwara zvakanyanya kuti vaore pavanenge vakamirira kurapwa. Nekuda kweizvozvo, autologous cell kurapwa vagadziri vakaisa yakakosha pakudzikisa nguva pakati pekutorwa kweropa uye yakagadziridzwa cell re-infusion, inozivikanwawo sevein-to-vein nguva.
Ongororo yepamberi yekiriniki yakaburitswa muNature Biomedical Engineering yakaratidza kuti huwandu hwenguva, zvinhu, uye basa rinodiwa kugadzira CAR T masero zvinogona kudzikira zvakanyanya. Maererano nevatsvakurudzi, izvi zvinogona kunyanya kubatsira kune vanhu vane zvirwere zvinokurumidza kufambira mberi uye munzvimbo dzechipatara dzisina zviwanikwa.
“While traditional manufacturing approaches for creating CAR T cells that take several days to weeks continue to work for patients with ‘liquid’ cancers like leukaemia, there is still a significant need to reduce the time and cost of producing these complex therapies,” said Dr. Michael Milone, an associate professor of pathology and laboratory medicine and one of the study’s co-leaders. The manufacturing method reported in this study is a testament to the potential to innovate and improve the production of CAR T masero ekurapa for the benefit of more patients, building on our research from 2018 that reduced the standard manufacturing approach to three days, and now to less than 24 hours.
Vatsvagiri vakawana kuti mhando yechigadzirwa cheCAR T cell, kwete nhamba yayo, ndiyo yakakosha mutyairi wekubudirira kwayo mumhando dzemhuka. Tsvagiridzo yavo yakaratidza kuti nhamba diki yemhando yepamusoro CAR T masero akagadzirwa kunze kwemuviri pasina kuwedzera kukuru aidiwa kune nhamba huru yeakaderera-mhando masero eCAR T anowedzerwa zvakanyanya asati adzokera kumurwere.
T masero anofanira kushandiswa nenzira inoita kuti awedzere uye awedzere kuitira kuti ashandiswe mumaitiro ekugadzira echinyakare. Kushandisa nzira dzeinjiniya dzakabva muchikamu pakunzwisisa kuti HIV inotapurira sei masero eT, vaongorori vePenn vakakwanisa kubvisa danho iri rekugadzira. Chikwata chakawana nzira yekuendesa majini akananga kune asina-activated T masero achangobva kutorwa muropa. Izvi zvakapa bhenefiti mbiri yekumhanyisa iyo yese yekugadzira maitiro vachiri kuchengetedza potency yeT masero. Iyi nzira haibvumire varwere kutapukirwa neHIV.
Kuwanikwa kwemurwere kumasero ekurapa kunogumira nekuda kwemutengo. Vatsvagiri vanotarisira kuti nekudzikisa mutengo uye nguva yakabatana nekugadzira, marapirwo aya anogona kuitwa kuti adhure zvakanyanya, achibvumira varwere vazhinji kuwana iwo.
“Iyi nzira itsva inoshamisa pakuti inogona kubatsira varwere vangazotadza kubatsirwa kubva CAR T cell kurapa, sevaya vane kenza iri kukurumidza kufambira mberi, nekuda kwenguva yakakosha inodiwa kugadzira marapirwo aya, "akadaro Dr. Saba Ghassemi, purofesa mubatsiri wepathology nemishonga yerabhoritari uye mumwe mutungamiri wezvidzidzo. "Kunyatsogadzirisa hurongwa hwemasero eT ane CAR mukati meawa 24 mune imwe nzira yakapusa yekugadzira isina T cell activation kana yakakosha kunze-ye-muviri tsika inovhurawo sarudzo yekuwedzera kupi uye riini marapiro aya anogadzirwa." Kwete chete inogona kuwedzera huwandu hwenzvimbo yekugadzira yepakati, asi kana iri nyore uye isingachinjiki zvakakwana, zvinogoneka kugadzira marapirwo aya munharaunda pedyo nemurwere, kugadzirisa matambudziko mazhinji ehurongwa anotadzisa kupihwa kwekurapa kunoshanda uku, kunyanya muhurombo. nharaunda.”
Vatsvagiri vakati kudzidza kwavo "inzira yekuwedzera tsvakiridzo yekiriniki kuti vanzwisise kuti masero eCAR T anoshanda sei muvarwere vane mamota chaiwo vachishandisa zano rakapfupikiswa."
Mukubatana neNovartis neChipatara cheVana chePhiladelphia, Penn nyanzvi dzakatungamira tsvagiridzo, budiriro, uye miedzo yekiriniki yekurapa uku kweCAR T kunotyisa. Novartis akapa mvumo kune imwe tekinoroji inoshandiswa mukuferefeta uku kubva kuYunivhesiti yePennsylvania.
