Cases of NK cell immunotherapy for advanced liver cancer
92-year-old advanced cancer patient has complete remission
Seven years ago, Ms. M, who was in her 80s, was diagnosed with hepatocellular carcinoma (caused by cancer in the past due to blood transfusion and infection with hepatitis C virus). Because she was in good health at the time, she was successfully surgically removed.
In 2016, the cancer recurred, but considering that Ms. M was already old at this time, surgery was no longer recommended.
Ms. M. was born into a medical family, and her daughter is also a doctor. She consulted on many treatments suitable for mothers other than surgery. Due to the poor location of the шишик, radiofrequency and embolization treatments are not feasible. Among the most cutting-edge radiotherapy methods, the first is to exclude heavy ions, which are not applicable at all, and the second is протон терапия, and the mother ’s physical strength is unbearable. Finally, only the TOMO knife can accurately locate the mother ’s complex disease. Irradiation.
Doctors suggest that Ms. M should be treated conservatively at such an old age. You can try a mild systemic treatment-NK cell reinfusion to improve immunity without using the toxic and side effects of drug treatment. At the same time, combined with Tom knife radiotherapy to kill рак клеткалары.
The high activation period of NK cell reinfusion into the body is 2-3 days, so Ms. M performs NK cell reinfusion on the weekend after local radiotherapy with TOMO knife. NK cells can move with the blood throughout the body to achieve local + systemic treatment synergy Better treatment effect with anti-cancer effect.
After treatment, ultrasound and PET-CT examination showed that the patient’s lesion was in a radical state. The local lesion has disappeared, the patient’s worry about the metastasis has not appeared, there is no jaundice and ascites, and the patient’s body gradually recovered.
NK cell systemic anti-cancer therapy
NK (natural killer cell, NK) is the immune cell with the strongest anti-cancer effect. The most powerful thing is that it does not require the antigen presentation process and does not require other immune cells to regulate. It can take the initiative, direct, and quickly remove foreign bodies (Virus bacteria infect cells, cancer cells, senescent cells, etc.) Local Fa-rectification.
Although they can quickly defend and directly attack tumor cells, unfortunately, NK cells are only a small part of the immune system, accounting for only 10% of white blood cells. And the study found that after 25 years of age, human immunity declines and the number of NK cells becomes less. The number and activity of NK cells in tumor patients and patients after tumor surgery have changed to a certain extent, and they cannot effectively exert anti-cancer effects..
Изилдөөчүлөр азыр "асырап алуучу" NK клетка терапиясына көңүл буруп жатышат - жакын донорлордон NK клеткаларын чогултуп, аларды бейтаптарга сайып жатышат. Бул коопсуз экени далилденген жана Т-клетка терапиясынан айырмаланып, NK клеткалары реципиенттин кыртыштарында трансплант-каршы-хост оорусуна алып келбейт.
In order to improve the activity and number of NK cells in the body, Japanese scientists have invented a multiplier method, which is to extract 50ml from human blood, isolate a small amount of NK cells and then expand the culture to increase the number to the original 1000 times, the number reaches 1 billion to 5 billion, and then returned to the body, a large number of NK cells will circulate 3000 to 4000 times with the blood throughout the body, killing cancer cells, aging cells, diseased cells, bacteria and viruses in the body Once again, to achieve the purpose of anti-cancer and anti-cancer, improve immunity and prolong survival.
At present, there is a large amount of evidence that immunocytotherapy (NK cells) before and after the surgery period can reduce the risk of recurrence and metastasis.
Why liver cancer patients are more suitable for NK cell therapy
NK cells, like the “Molecular Patrol”, patrol the bloodstream everywhere. Once they find foreign cells or mutant cells that have lost their self-identification (called MHC), the NK cell’s receptor immediately sends a signal and rushes to the target cell membrane That is to say, the NK cells must be on the front line of the battle. It releases toxic particles to it, quickly dissolves the target cells, and causes the cancer cells to die within 5 minutes.
It should be noted that NK cells, as the core part of the immune system, are the most valuable innate immune cells in the human body, but they are very few in human peripheral blood, accounting for only 5% -10% of lymphocytes. In the liver, NK cells account for 30-50% of lymphocytes.
Compared with circulating NK cells, NK cells in the liver have unique phenotypic characteristics and functional characteristics, showing higher cytotoxicity to tumor cells.
During the occurrence of боор рагы, the proportion of NK cells and the function of cytokines (interferon-γ) production and cytotoxic activity are reduced.
NK клеткалары рак клеткаларын кантип өлтүрөт?
The reason why NK cells can kill cancer cells is because of the balance of the signals of activated receptors and inhibitory receptors on the surface of NK cells. Activated receptor molecules are expressed on the surface of cancer cells and infected cells and recognized by NK cells. In addition, cancer cells and infected cells often lose MHC I molecules, making them susceptible to NK cell recognition.
NK cells exist in human blood and are “first responders.”. It is like a policeman who has been on duty in the body. As blood runs around, NK cells continuously contact other cells while patrolling. Once an abnormality is found in the body Cells, immediately stable, accurate, ruthlessly wait for a time to deal with. They attack and release cytotoxic particles containing perforin and granzyme on the target cell membrane before T cells are deployed, triggering the self-destruction of cancer cells. They can also eliminate cancer stem cells circulating in the body and help prevent metastasis.
NK клетка терапиясынын артыкчылыктары
1. Иммундук клетка терапиясы хирургия, химиотерапия жана радиотерапиядан кийинки төртүнчү дарылоо ыкмасы болуп саналат. NK клетка терапиясы радиотерапия жана химиотерапия менен бирге хирургиялык жол менен толугу менен жок кылынган шишик клеткаларын эффективдүү алып салышы мүмкүн;
2. NK клетка терапиясы радиохимиотерапия менен айкалышып, радиохимиотерапиянын эффективдүүлүгүн жакшыртат жана терс таасирлерин азайтат;
3. Хирургиялык же радиотерапия жана химиотерапия үчүн ылайыктуу эмес өнүккөн рак бейтаптар үчүн, NK клетка терапиясы жакшы тандоо болуп саналат;
4. Операциядан кийин NK клеткалары менен үзгүлтүксүз дарылоо рактын кайталанышын жана метастаздарын алдын алат;
5. Рак оорусун жеңилдетүү, уйкуну жакшыртуу, пациенттин жашоо сапатын жакшыртуу жана пациенттин жашоо циклин узартуу;
6. Суб-ден соолугу чың адамдар үчүн NK клетка терапиясын колдонуу рактын рискин азайтат.
Which patients are suitable for NK cell therapy?
Patients with poor physique before surgery, slow recovery after surgery, and fear of occult cancer cells not being completely eradicated.
After radiotherapy and chemotherapy, immunity is low, and side effects are obvious (such as loss of appetite, nausea, hair loss, skin inflammation, etc.), and patients who expect to increase the effect of chemoradiation.
Радиотерапия жана химиотерапиянын терс таасирлеринен чочулагандыктан, терапиялык эффекттерге жетишүү үчүн ар кандай дарылоону колдонгонго үмүттөнгөн пациенттер.
Cancer cells in advanced tumors have spread throughout the body, but conventional treatment methods have been powerless, and patients who expect to prolong survival and improve quality of life.
NK cell therapy treatment process
1. Кан алуу
Draw 30-50ml of peripheral blood o
f cancer patients and extract mononuclear cells;
2. Laboratory cultivation
In the laboratory, conduct NK cell induction and expansion for 5-7 days;
3. Кайтып келүү
NK клетка культурасы аяктагандан кийин, ал инфузия сыяктуу рак оорулууга кайтарылат.
